In vitro production of antioxidants of naringin and hesperidin and their activity in unsaturated fatty acid for oil preparation pharmaceuticals
Zarina Zakaria
School of Bioprocess Engineering Universiti Malaysia Perlis
Abstract:
The use of natural antioxidants in pharmaceuticals has been restricted by the limited natural resources available. Plant as the main sources of these valuable compounds, is inconsistent supply as more land were developed and tremendous climate change all over the world. To produce in vitro antioxidant compounds is making possible with the emergence of tissue culture technique. Research on young pomelo (Citus grandis) fruit has successfully produced callus tissues that contain the antioxidant compounds. Analysis by HPLC found that naringin and hesperidin are the major composition of total flavonoids which detected as 250.6 mg/l and 2.7 mg/l respectively when the callus was grown on media supplemented with 2250 mg/l nitrate, 535 mg/l phosphate and 35 g/l sucrose for 7 days. Tested on unsaturated fatty acid proved that antioxidants compound in the callus did has an antioxidant activity. Therefore callus from pomelo may be applied in oil preparation of pharmaceutical products as alternative for plant origin antioxidants.
